I would say that the play is probably not THAT much better, but you will probably have 1-2 more experienced players at the table and in general have more players that have already moved through two levels of experience to get to the 2/4 level.
I have found that the lowest limit on a site is by far the easiest to beat, as you move up (even from 50/1 --> 1/2) the play tightens "A LITTLE" and you have to be a little more careful.
Mathbabe, if you are losing at 50/1, where you can make a lot of mistakes and still have other peoples more numerous mistakes compensate, then you should use Pokertracker to try to figure it out and FIX whatever is wrong at 50/1 before you move up, IMO.
At 50/1 rake should not even be an issue because you should be winning enough that the worst players at the table will be paying your share as well as their own.
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